“
“Among other factors, a distinct gene redundancy is discussed to facilitate high metabolic versatility of rhodococci. selleck chemical Rhodococcus opacus 1CP is a typical member in that respect and degrades a multitude of (chlorinated) aromatic

compounds. In contrast to the central pathways of aromatic degradation in strain 1CP, little is known about the degree of gene redundancy and to what extent this is reflected on protein level within the steps of peripheral degradation. By means of degenerated primers deduced from tryptic peptides of a purified phenol hydroxylase component and using the amplified fragment as a labelled probe against genomic 1CP-DNA, three gene sets encoding three different two-component phenol hydroxylases pheA1/pheA2(1–3) could be identified. One of them was found to be located on the megaplasmid p1CP, which confirms the role of these elements for metabolic versatility. Protein chromatography of phenol- and 4-chlorophenol-grown 1CP-biomass

gave first evidences on a functional expression of these oxygenases, which could be initially characterised in respect of their substrate specificity. “
“In the paper by Sambir et al. (2011), the Acknowledgements section did not properly list the NIH grant. It should have read: M.S. and L.B.I. contributed equally ATM/ATR inhibitor cancer to this work which was supported by NIH grant R01 AI 048856 to F.C.C. We would like to thank Dr M. Norgard, University of Texas Southwestern

Medical Center, Dallas, TX, for providing B. burgdorferi 297, clone BbAH130, and Dr Julia Bugrysheva for advice. The accepted version of the article is now available on PubMed Central. “
“Xylella fastidiosa causes a serious Pierce’s disease (PD) in grapevine. Xylella fastidiosa cells from a PD strain were grown in a pure xylem fluid of a susceptible grapevine cultivar vs. xylem fluid from citrus, which is not Methane monooxygenase a host for this strain of X. fastidiosa. When grown in grapevine xylem fluid, cells of the PD strain formed clumps and biofilm formed to a greater extent than in citrus xylem fluid, although the PD strain did grow in xylem fluid of three citrus varieties. The differential expression of selected genes of a PD X. fastidiosa strain cultured in the two xylem fluids was analyzed using a DNA macroarray. Compared with citrus xylem fluid, grapevine xylem fluid stimulated the expression of X. fastidiosa genes involved in virulence regulation, such as gacA, algU, xrvA, and hsq, and also genes involved in the biogenesis of pili and twitching motility, such as fimT, pilI, pilU, and pilY1. Increased gene expression likely contributes to PD expression in grapevine, whereas citrus xylem fluid did not support or possibly suppressed the expression of these virulence genes.