We present changes in the characteristics studied, calculated per genet containing several ramets formed during the vegetative growth. In the last year of observation, we observed disintegration of genets and formation of clone selleck inhibitor clusters. Despite this fact, we defined the units as genets, that is, genetic individuals containing some ramets.2.3. Measurements under Field ConditionsEach summer, at the peak of flowering, 30 individuals per each mode of plant establishment were measured when available. Several vegetative and reproductive characters were measured: the total number of ramets per genet (TNR), the number of flowering ramets per genet (NFR), the height of flowering stems per genet (HS), the number of heads per genet (NH), the number of heads per flowering stem of the genet (NHFS), and the head diameter per genet.

The total area of heads per genet (AH) was calculated on the basis of the head diameter. On the basis of 6-year observation, the cumulative area of heads per genet (CAH) and the cumulative number of heads per genet (CNH) were determined. The morphological and reproductive parameters were recorded from 30 plants/repetition.The survival time was assessed by recording survival of individual plants each June from the beginning of the experiment until July 2012. A plant was considered dead when it was not found during subsequent censuses. The presented climatic data were obtained from the Felin climatic station.2.4. Statistical AnalysisPrior to the analysis, all data were tested for normality with the Shapiro-Wilk test. Variance heterogeneity was checked using a Levene?s test.

Parametric data were tested using one-way analysis of variance (ANOVA) with subsequent Tukey tests (data were normally distributed with homogeneous variances). When the data were not normally distributed or the variance was not homogeneous, the differences between the 3 modes of introduction were tested using Kruskal-Wallis tests with subsequent Mann-Whitney U-tests. All results are expressed as Batimastat means �� SD, and the differences were considered significant P < 0.05. All statistical analyses were carried out using the Statistica software programme.3. ResultsThe total number of ramets per genet (TNR) differed significantly between the three different modes of plant establishment in each study year (Table 1, Figure 2). In the first full vegetation season (2007), plants introduced as CS formed 2�C6 new offspring ramets (mean 5.4), and genets introduced as RS formed 3�C8 new offspring ramets (mean 6.4). However, individuals grown from seeds produced 2�C5 new offspring rosettes (mean 3.4). In the second year of observation, the CS genets had a higher TNR, while in the third year, the RS were characterised by a higher TNR.