brasiliensis. The nucleotide and amino acid positions are marked on the left side. Lower case letters represent the untranslated 5′ region. Bold letters in nucleotide sequence represent the start and stop codons. Two introns were found in the genomic sequence

and are shown in italic. Three conserved residues (marked with arrows) of amino acids (asparagine – D; histidine – H and serine – S) belonging to the active site of serine proteases from the subtilase family S08 are evidenced. Six putative AZD1152 in vitro N-glycosylation sites are marked in bold letters. A signal peptide formed by the first 16 amino acids is underlined. The TATA box in the promoter region is evidenced by white letters. A GATA binding region of the transcription factor AreA was found and is evidenced by a white box. (JPEG 270 KB) References 1. Rawlings ND, Barrett AJ: Evolutionary families of peptidases. Biochem J 1993, 290:205–218.PubMed 2. Jousson O, Lechenne B, Bontems O, Mignon B, Reichard U, Barblan J, Quadroni M, Monod M: Secreted subtilisin gene family in Trichophyton rubrum . Gene 2004, 339:79–88.PubMedCrossRef 3. Baldo A, Tabart J, Vermout S, Mathy A,

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