2. Aralia Chinesis L simultaneously suppress the proliferation of collagen fibers in the liver tissue in order to reduce the degree of liver fibrosis in rats. and at the same time with the suppression of protein expression of a-SMA. Key Word(s): 1. Aralia Chinesis L; 2. Hepatic fibrosis; 3. cytokines; 4. Apoptosis; Presenting Author: GANG ZHAO selleck inhibitor Additional Authors: LEI DONG, HONG LI, HAITAO SHI, XIAOLAN LU Corresponding Author: GANG ZHAO Affiliations: Xi′an Jiaotong University; [email protected] Objective: To observe the expression of fatty acid synthase (FASN) in alcohol-induced liver injury in mice, and investigate the possible mechanism of
EGFR in the process. Methods: Primary mice hepatocytes were cultured conventionally.
Four groups included normal group (saline group), ethanol group, ethanol plus EGFR tyrosine kinase inhibitor (Gefitinib) group and Gefitinib alone group were set up randomly. Total RNA and protein from liver cells were extracted, and real time-PCR and western-blot were applied to measure the gene and protein expression of FASN, Sterol regulatory element binding proteins (SREBP-1c) and EGFR. Results: Normal liver cells only slightly expression of FASN. After treated with Gefitinib, FASN expression in liver cells was no significant difference between normal liver cells. After treated with ethanol, FASN, SREBP-1c and EGFR expression both in gene and protein levels Selleck Alpelisib were significantly increased in liver Cediranib (AZD2171) cells. In ethanol plus Gefitinib group, expression levels of FASN and SREBP-1c were significantly lower, EGFR mRNA expression was still in the high value, while its protein expression was significantly decreased.
Conclusion: FASN slightly expresses in normal liver cells, but has a high abundance expression in alcohol-induced liver injury, maybe EGFR signaling pathway plays an important role during the process. Key Word(s): 1. EGFR; 2. Liver injury; 3. Fatty acid synthase; 4. TKI; Presenting Author: JOHNC HSIANG Additional Authors: WAYNE BAI, ARLO UPTON, ED GANE, STEPHEN GERRED Corresponding Author: JOHNC HSIANG Affiliations: Middlemore Hospital Objective: Local guidelines recommend six monthly hepatic ultrasound (US) and alpha –fetoprotein (AFP) testing for patients with a high risk of developing hepatocellular carcinomna (HCC). To assess the adherence to HCC surveillance guidelines at our institution. Methods: Patients with a high risk of HCC between 2007 and 2011 were identified from our clinic database. A retrospective review of electronic records was undertaken to record clinic attendance and adherence to six monthly AFP and US surveillance. Results: A total of 460 patients were identified. Cirrhosis was present in 409, severe hepatic fibrosis in 38, chronic hepatitis B and a family history of HCC in 13.