3% from the LXR peaks contained such a DR4 form RE. Also motifs for your transcription components PU. one and AP 1 have been co enriched, The capability of LXRs to integrate lipid metabolic process and immune functions places them in a perfect position to tailor macrophage responses. Consequently, we performed a genome wide evaluation of T09 induced LXR binding in macrophage style phorbol selleckchem myristate acetate dif ferentiated THP 1 human monocytic leukemia cells by ChIP Seq applying a remarkably distinct anti LXR antibody. In complete we identified 1357 chromosomal LXR binding loca tions, of which 526 had been observed immediately after the T09 deal with ment. On the mRNA level the ligand induced 1258 genes and repressed 455 genes. We studied the LXR cis trome and identified LXR enriched genomic areas at the same time as individual LXR target genes.
Binding of LXR is targeted on 112 genomic areas with personal constel lations Rutin of binding web pages and target genes. We could con firm that LXR controls functions connected to lipid metabolic process and observed a strong hyperlink to apoptosis. Therefore, this initial report on genome wide binding of LXR in the human cell line provides new insights into LXR target genes and their link to physiological pro cesses, for example apoptosis.