875, P=0.000). The mRNA levels of Pyk2 and FAK detected by quantitative real time RT�CPCR were consistent with the intracellular protein expression. Significantly higher mRNA levels of Pyk2 and FAK were found in the tumour tissues of the patients with inhibitor Lenalidomide higher protein expression of Pyk2 and FAK (Figure 4). Figure 1 Intracellular protein expression of Pyk2 (A) and FAK (B). Representative intracellular expression of Pyk2 was found both to be located in the cytoplasm (A1) nuclear of tumour cells (A2). The positive signals of FAK were found in the cytoplasm of tumour … Figure 2 Different expression patterns of Pyk2 (A) and FAK (B) by immunostaining. There were 59.2% (29 out of 49) of patients with higher intracellular expression levels of Pyk2 in tumour tissues (A1-a/c), whereas 40.8% (20 out of 49) of .

.. Figure 3 Different protein expression patterns of Pyk2 (A) and FAK (B) by western blot. PYk2 protein expression in different HCC cell lines (C) and subcellular fractionation of Pyk2 in PLC and MHCC97L cell lines (D) by Western blot. T: tumour; NT non-tumour. 1: … Figure 4 mRNA expression of Pyk2 (A), FAK (B), Ezrin (C) and fibronectin (D) in tumour and non-tumour tissues by real-time RT-PCR of patients with higher or lower protein expression of Pyk2 or FAK, respectively. *: Higher expression vs lower expression: … Gene expression of ezrin and fibronectin in HCC patients with higher or lower protein expression of Pyk2 To examine the correlation of Pyk2 with other potential metastatic genes, we detected the mRNA levels of ezrin and fibronectin in the liver tumour and non-tumour tissues.

The gene expression levels of ezrin and firbronectin in liver tumour tissues were found consistent with their protein expression level of Pyk2 (Figure 4). Patients with higher protein/gene expression levels of Pyk2 also had higher mRNA levels of ezrin and fibronectin. Correlation of the expression Drug_discovery of Pyk2 and FAK with clinicopathological parameters: overexpression of Pyk2 was significantly associated with poor survival and tumour invasiveness Patient survival and pathological data were analysed between the patients with higher or lower protein expression of Pyk2 and FAK in tumour tissues. The higher intracellular protein expression level of Pyk2 was significantly correlated with larger tumour size (P=0.005), advanced new Edmonson’s staging (P=0.033) and venous invasion (P=0.02) (Table 1). Similar to the results of Pyk2, overexpression of FAK also contributed to larger tumour size (P=0.025) and advanced new Edmonson’s staging (P=0.017) (Table 2). However, there was no difference in the incidence of venous invasion and advanced tumour-node-metastasis staging between patients with higher and lower expression of FAK.