Fig. S4. Venn diagrams comparing (a) the phylotype numbers and (b) the Chao1 species richness estimates in the archaeal clone libraries HO28S9 and HO28S21. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. “
“Magnaporthe oryzae germlings tightly attach to the host surface by producing extracellular matrix (ECM) from germ tubes and

appressoria, which see more are important for the early infection process. To understand the adhesion mechanisms of ECM during differentiation of infection structure, we evaluated the effects of various enzymes on M. oryzae germlings and the disease selleck products symptoms

of the host plant, wheat. Treatment with β-mannosidase, collagenase N-2, collagenase S-1, or gelatinase B at 1-h postinoculation (hpi) resulted in germling detachment, although producing normal appressoria. Treatment with matrix metalloproteinases (MMPs) at 6 hpi also caused germling detachment. Furthermore, we confirmed by the inoculation tests and scanning electron microscopy that the germlings on the wheat plant were removed and did not manifest pathogenicity on treatment with MMPs. The most effective MMPs were crude collagenase, collagenase S-1, and gelatinase B, suggesting that the application of MMPs is promising for crop protection from fungal diseases by its detachment action. Magnaporthe oryzae, a pathogen of a wide variety of cereal crops including barley, rice, and wheat, causes significant yield loss (Ou, 1985). This pathogen disseminates via asexual spores and propagates exponentially. When these asexual spores land on plant surfaces and absorb water, spore tip mucilage (STM) is secreted from an apical compartment in the spore, making the spore attach to the surfaces (Hamer et al., 1988). The

Rebamipide attached spore elongates the germ tube and then differentiates into the specific infection machinery, the appressorium, which elaborates the penetration peg at the bottom and generates enormous turgor, passing through the rigid plant cuticle (Howard et al., 1991; Howard, 1994). Therefore, the germlings of the spores (infection structures) need to withstand the counteracting pressures (i.e. turgor and penetration force) on the plant surface. Extracellular matrix (ECM), abundantly secreted from germ tubes and appressoria, seems to be essential for adhesion and penetration and is therefore regarded as a pathogenicity factor (Apoga et al., 2001; Inoue et al., 2007; Schumacher et al., 2008). Up to now, several control measures have been used to control blast disease. Identification of race-specific or broad-spectrum resistance genes enables breeders to develop new cultivars (Roumen, 1994).