Mol Microbiol 2013, 87:1074–1087 PubMedCrossRef 33 De Pedro MA,

Mol Microbiol 2013, 87:1074–1087.PubMedCrossRef 33. De Pedro MA, Quintela JC, Holtje JV, Schwarz H: Murein segregation in Escherichia coli. J Bacteriol 1997, 179:2823–2834.PubMed 34. Reusch RN: Insights into the structure and assembly of Escherichia coli outer membrane protein a. FEBS J 2012, 279:894–909.PubMedCrossRef 35. Spector J, Zakharov S, Lill Y, Sharma O, Cramer

this website WA, Ritchie K: Mobility of BtuB and OmpF in the Escherichia coli outer membrane: implications for dynamic formation of a translocon complex. Biophys J 2010, 99:3880–6.PubMedCrossRef 36. Ritchie K, Spector J: Single molecule studies of molecular diffusion in cellular membranes: determining membrane structure. Biopolymers 2007, 87:95–101.PubMedCrossRef 37. Sambrook J, Russel DW: Molecular cloning: a laboratory manual. Third edition.

Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press; 2001. 38. Adiciptaningrum AM, check details Blomfield IC, Tans SJ: Direct observation of type 1 fimbrial switching. EMBO Rep 2009, 10:527–32.PubMedCrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions All authors conceived the study, designed the experiments and participated in data analysis and interpretation. GSV carried out the experiments and drafted the manuscript. All authors read and approved the final manuscript.”
“Background Methicillin-resistant staphylococci represent a great challenge for treatment and public health. In staphylococci, methicillin resistance is mainly due to the expression of the mecA gene, which specifies penicillin binding protein 2a (PBP2a), a transpeptidase with a low affinity for β-lactams [1, 2]. mecA is carried by a mobile genetic element (MGE) termed the staphylococcal cassette chromosome mec

(SCCmec) [2, 3]. Generally, SCCmec contains two essential components, i.e. the mec gene complex and the ccr gene complex. The mec gene complex consists of mecA, the regulatory genes and associated insertion sequences and has been classified into six different classes, i.e. A, B, C1, C2, D and E. Cassette chromosome recombinase (ccr) genes (ccrC or the pair of ccrA and ccrB) encode recombinases mediating integration and excision of SCCmec into and from the chromosome [2, 3]. The ccr gene(s) Galeterone and surrounding genes form the ccr gene complex. A Staphylococcus haemolyticus clinical isolate, WCH1, was found carrying mecA but no ccr genes. Although clinical isolates of S. haemolyticus containing mecA but lacking ccr genes have been reported previously [4–6], SU5416 information about the detailed contexts of mecA is largely absent. The genetic context of mecA in WCH1 was therefore investigated using long-range PCR, PCR mapping, inverse PCR and sequencing as described previously [7]. Results and discussion The minimum inhibitory concentration (MIC) of cefoxitin against WCH1 was 128 μg/ml.

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