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“Objective: To ensure appropriate allocation of research funds, we need methods for identifying high-priority research needs. We developed and pilot tested a process to identify needs for primary clinical research using a systematic review in gestational diabetes mellitus.
Study Design and Setting: We conducted eight steps: abstract research gaps from a systematic review using the Population, Intervention, Comparison, Outcomes, and Settings (PICOS) framework; solicit feedback from the review authors; translate gaps into researchable questions using the PICOS framework;
solicit feedback from multidisciplinary stakeholders at our institution; establish consensus among multidisciplinary Vorinostat clinical trial external stakeholders on the importance of the research questions using the Delphi method; prioritize outcomes; develop conceptual models to highlight research needs; and evaluate the process.
Results: We identified 19 research questions. During the Delphi method, external stakeholders established consensus for 16 of
these 19 questions (15 with “”high”" and 1 with “”medium”" clinical benefit/importance).
Conclusion: We pilot tested an eight-step process to identify clinically important research needs. Before wider application of this process, it should be tested using systematic reviews of other Pfizer Licensed Compound Library diseases. Further evaluation should include assessment of the usefulness of the research needs generated using this process for primary researchers and funders. (C) 2013 Elsevier Inc. All rights reserved.”
“Green coffee oil (GCO) is a potential natural product for application in health care products. In this study, the properties of GCO from spent coffee grains were evaluated aiming its application in sunscreens. GCO showed an intense synergistic effect in SPF value when it was
associated with the synthetic sunscreen ethylhexylmethoxycinnamate, leading to an increase of 20% in SPF. Additionally, the antioxidant activity, the cytotoxicity and the sun protection factor (SPF) of GCO were determined. The results show no cytotoxic effect for skin and liver cells in vitro, even at high concentrations. The results suggest the use of GCO as a potential natural product for sunscreens formulations to improve the efficiency and protection against LXH254 datasheet skin damage. (C) 2013 Elsevier B.V. All rights reserved.”
“Purpose Endothelial progenitor cells (EPCs) are a promising cell source for vascular tissue engineering approaches in surgery. Diverse biomaterials have been used as matrices for EPC cultivation. In this in vitro study, fibrin in combination with growth factors was examined as an optimized culturing scaffold for EPCs.
Methods EPCs were isolated from peripheral blood by density gradient centrifugation and positive selection using CD34-specific magnetic beads. Cells were seeded on fibrin for 3, 5, and 10 days or on fibronectin for control purposes.