Peaks below a fluorescence threshold level of 50 were excluded except where a clear trend of same t-RF was detected in other
samples. Estimation of relative quantity of P. phosphoreum was done by calculating the ratio of its peak area to the total peak area generated in the chromatogram. Statistical analysis of t-RFLP profiles The relative abundance of each t-RF in the profile was calculated by dividing the respective peak area of each t-RF with the Pevonedistat in vivo total peak area generated between 50-600 base pairs. The profiles from different combinations of labelled primers and restriction enzymes were all combined in one dataset for principal component analysis (PCA) to enhance the analytical power of the model. PCA of t-RFLP profiles from different fish samples was performed using the Unscrambler version 9.5 (Camo ASA, Oslo, Norway). The data was not weighed in order to maintain the ability of t-RFLP to quantitatively discriminate between peaks, representing different taxonomic units. Full cross validation was used. Gas Chromatography-Mass Spectrometry Air and MAP LS samples stored at -2°C were analysed at the beginning, mid- and at the end Olaparib supplier of storage. About
175 g of fish fillets were cut in pieces and dispersed evenly on a sampling dish (plastic tray). Measurements and identification of volatiles was done according to Olafsdottir et al. . Acknowledgements This work was supported by the AVS Funds of the Ministry of Fisheries in Iceland, the Icelandic Center for Research (ICR) and the European Commission through the Chill-On Integrated Project (FP6-016333-2). The authors would also like to thank Professor Jeffrey Hoorfar for his help in the manuscript preparation. References 1. Olafsdottir G, Lauzon HL, Martinsdottir E, Oehlenschlager J, Kristbergsson K: Evaluation of shelf-life of superchilled cod ( Gadus morhua ) fillets and influence of temperature fluctuations on microbial and chemical quality indicators.
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