The ultrastructural analysis revealed that the acrosome was better preserved after freezing in the SOD 100 U/ml and GSH 2 and 5 mM (p < 0.05) groups than the other groups, whereas mitochondria small molecule library screening in both the control group and the 7 mM GSH group suffered the greatest damage. The plasma membrane remained preserved after freezing, regardless of the group. For in vivo fertilization, the SOD group achieved better results than the GSH group (p > 0.05). It can therefore be concluded that the addition of SOD 100
U/ml and GSH 2 and 5 mM preserves the acrosome integrity of frozen ram spermatozoa, while the addition of SOD 100 U/ml to Tris egg-yolk extender offers protection to the membranes of sperm cells after thawing.”
“Until recently, the sonographic visualization of pulmonary and pleural diseases was considered a poorly accessible method, due to the selleck products inability of sound to penetrate air-filled lung. Despite its limitations, lung ultrasonography is becoming an important diagnostic tool in a
growing number of pathological situations such as pneumonia, atelectasis, interstitial-alveolar syndrome, pulmonary embolism, pneumothorax and pleural effusion. The low sensitivity of CXR and the difficulties of performing CT make this technique invaluable for bedside use in the intensive care unit. Lung ultrasonography is an easily repeatable and radiation-free technique, and therefore, an attractive imaging tool for use on a daily basis, especially in the management of critically ill patients.”
“Purpose: To develop and evaluation ascorbyl palmitate niosomes in order to achieve a transdermal
and/or systemic nanocarier of doxycycline.
Methods: Vesicles were formed from ascorbyl palmitate in combination with cholesterol and a negatively charged lipid, dicetyl phosphate. Niosomes were prepared by film hydration method followed by sonication in which aqueous doxycycline solution (in phosphate buffered saline) was encapsulated in the aqueous regions of the vesicles. The vesicles were CDK inhibitor evaluated for entrapment and in vitro release as well as for their thermal properties and shape by ultraviolet spectroscopy (UV), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM), respectively. The effect of process conditions – sonication time, pH, hydration temperature and centrifuge speed – on niosome properties was investigated.
Results: DSC of pure lipids, vesicles dispersion and mixture of lipids confirmed the formation of niosomes. Other results show that >90% of drug was entrapped in the vesicles and the vesicles were spherical in shape. Drug release from the vesicles was slow (<60% after 8 h). Nanovesicle size was significantly (p < 0.05) affected by sonication time and hydration pH.