We are thus far from having fully elucidated the complex role of saliva on Candida species. In conclusion, to our knowledge, this study investigates for the first time the effect of saliva
on Candida growth in tap water. The survival ability of Candida could APO866 in vivo be influenced by the carbohydrates and proteins contained in saliva; however, d-glucose and total protein concentrations were very low in our saliva preparation (0.02 and 0.78 g L−1, respectively). Candida is rarely isolated from water but its persistence may be responsible of an infectious risk associated with the water from dental units, especially for the most fragile patients or dentists. We demonstrated the variable susceptibility of Candida yeasts in tap water, depending on the species. The results presented here highlight the positive influence of saliva on the growth of three species of Candida, saliva enabling the yeasts to survive and maintain their initial concentration in a poor environment such as tap water. In addition, CFU counts showed that saliva
enabled C. albicans and C. parapsilosis yeasts to grow significantly. So, Candida yeasts from the human oral cavity, surviving in tap water because of the presence of saliva, could attach to a biofilm previously developed on the DUWL surface and continue its growth in this protected environment. The yeasts which then detach from the biofilm could contaminate other patients as well as dental this website staff running the dental unit. This could be a health risk for susceptible patients treated for dental care. Further studies are in progress to investigate the fate of yeasts in DUWL. “
“Department of Microbial Pathogenesis, Yale University School most of Medicine, New Haven, CT, USA SicA functions both as a class II chaperone for SipB and SipC of the type III secretion system (T3SS)-1 and as a transcriptional cofactor for the AraC-type
transcription factor InvF in Salmonella enterica subsp. enterica serovar Typhimurium. Bioinformatic analysis has predicted that SicA possesses three tetratricopeptide repeat (TPR)-like motifs, which are important for protein–protein interactions and serve as multiprotein complex mediators. To investigate whether the TPR-like motifs in SicA are critical for its transcriptional cofactor function, the canonical residues in these motifs were mutated to glutamate (SicAA44E, SicAA78E, and SicAG112E). None of these mutants except SicAA44E were able to activate the expression of the sipB and sigD genes. SicAA44E still has a capacity to interact with InvF in vitro, and despite its instability in cell, it could activate the sigDE operon. This suggests that TPR motifs are important for the transcriptional cofactor function of the SicA chaperone. “
“The transcription factor CsgD plays a key role in the control of biofilm formation in Escherichia coli by controlling the production of curli fimbriae and other biofilm components.