2009), chloropupukeanolides A and B (Liu et al. 2010), likewise isolated from the same fungus. The absolute configuration of 23 was assigned by X-ray crystallography and those of 24 and 25 by quantumchemical CD calculations. Biogenetically, chloropupukeanolides C-E (23–25) are presumably derived from the

oxidation-induced Diels-Alder reaction pathway as the known chloropupukeananin (Liu et al. 2008), chloropestolide A, chloropupukeanolides A and B, and chloropupukeanone KU55933 price A (Liu et al. 2010), via the putative biosynthetic precursors iso-A82775C and pestheic acid (Liu et al. 2008). The new metabolites 23–25 were tested for their cytotoxicity against two human tumor cell lines including epithelial carcinoma (HeLa) and colon adenocarcinoma (HT29) cells. Compounds 23 and 24 showed significant cytotoxicity against both cell lines, with IC50 values ranging from 1.2 to 7.9 μM, with a higher activity Verubecestat than the known positive control 5-fluorouracil, which gave IC50 values of 10.0 and 15.0 μM (Liu et al. 2011).

Annulosquamulin (26), a new dihydrobenzofuran-2,4-dione derivative, in addition to 10 known secondary metabolites, were isolated from the n-BuOH-soluble fraction of the endophytic fungus Annulohypoxylon squamulosum BCRC 34022, derived from the stem bark of the medicinal plant Cinnamomum sp. (Lauraceae) collected from Fu-Shan Botanical Garden, I-lan County, Taiwan. The structures of the isolated compounds were elucidated by means of 1D and 2D NMR {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| spectroscopy and by HRESIMS. Annulosquamulin (26) comprises a dihydrobenzofuran-2,4-dione skeleton, a 1-hydroxydecyl side chain, and a ɤ-lactone ring. The relative configuration of 26 was deduced from inspection of NOESY spectra, comparison with similar compounds, as well as by the help of the molecular modeling program CS CHEM 3D Ultra 10.0, with MM2 force-field calculations for energy minimization. Furthermore, 26 was evaluated for its in vitro cytotoxicity against MCF-7 (human breast adenocarcinoma), NCIH460 (non-small-cell lung cancer) and SF-268

(glioblastoma) cells by the MTT method ifoxetine with actinomycin D as positive control. 26 possessed moderate cytotoxicity against MCF-7, NCI-H460, and SF-268 cancer cell lines with IC50 values of 8.4, 8.9 and 6.5 μM, respectively (Cheng et al. 2012). Cultures of endophytic Alternaria tenuissima yielded a new isocoumarin, tenuissimasatin (27), together with 11 known compounds. The endophyte had been isolated from the bark of Erythrophleum fordii Oliver (Leguminosae), collected at Nanning, Guangxi Province, China. The new compounds as well as the known metabolites were identified by NMR spectroscopy and mass spectrometry. Furthermore, the absolute configuration of tenuissimasatin was obtained by CD calculation. All compounds were tested for their cytotoxic activities toward five human tumor cell lines, including intestinal epithelial (HCT-8), hepatoma (Bel-7402), gastric cancer (BGC-823), lung adenocarcinoma (A549) and ovarian cancer (A2780) cells.