Tukey’s honestly significant difference (HSD) was performed in the event of a significant F ratio. Two-tailed statistical significance was accepted at p < 0.05. When significant differences are stated, the mean difference plus the 95% confidence interval (CI) of the mean difference are provided . Results Acid-Base Balance There were
significant interactions (p < 0.01) and main effects for condition (p < 0.001) and time (p < 0.001) for all acid-base variables (pH, , & BE). Decomposition of the interactions indicated significant elevation in blood alkalosis for only the B condition when compared to both P and EG from 15 to 120 min during the ingestion period (Selleckchem Veliparib Figure 1). Across this time frame, mean differences between pH for the B and EG trials were 0.013 (smallest) to 0.045 (largest) with 95%CI ranging between 0.01 to 0.07. This distribution was similar between the B and P trials (mean difference between 0.010 (smallest) to 0.040 selleck kinase inhibitor (largest) with 95%CI ranging between 0.01 and 0.06). Following this profile, changes between B and EG trials ranged from the smallest Angiogenesis inhibitor mean difference of 1.6 mmol·L-1 to the largest of 4.3 mmol·L-1 (95%CI between 0.01 to 5.98 mmol·L-1), while B
and P trials followed a similar pattern (smallest mean difference = 1.3 mmol·L-1; largest mean difference = 4.2 mmol·L-1; 95%CI between 0.4 to 5.9 mmol·L-1). Finally, base excess changes between the B and EG trials ranged from the smallest mean difference of 3.8 meq·L-1 to the largest of 4.6 meq·L-1 (95%CI between 0.13 to 6.24 meq·L-1), while B and P trials again were similar (smallest mean difference = 2.4 meq·L-1; largest mean difference = 3.9 meq·L-1; 95%CI between 0.7 to 5.5 meq·L-1). Figure 1 Represented are the acid-base responses for
Energised Greens™ (9 g) (EG), 0.1 g·kg -1 BW sodium bicarbonate (NaHCO 3 ) or flour placebo (Placebo) conditions over 120 min Rho post ingestion. For all three acid-base variables, only the NaHCO3 condition resulted in significant elevation (*) in blood alkalosis between 15 and 120 min (p < 0.01) when compared to both Placebo and EG. GI Discomfort A large degree of intra-subject variability was evident in both the incidence and severity of GI discomfort (Figure 2). There were no significant interactions (p > 0.98) or main effects for condition (p > 0.80) or time (p > 0.57) for either incidence or severity. Figure 2 Represented in the following figure are mean ± SD scores for both incidence and severity of symptoms over 120 minutes after ingestion of either Energised Greens™ (9 g) (EG), 0.1 g·kg -1 BW sodium bicarbonate (NaHCO 3 ) or flour placebo (Placebo). Conclusions The aim of the current investigation was to profile the differences in acid-base response following both acute fruit and vegetable extract (EG) consumption and a standard, low dose of sodium bicarbonate. Our findings suggest that acute EG supplementation only induces minimal blood alkalosis (Figure 1).